From the degenerate nucleotide codes, only N is accepted. Please note that protein queries, with too many nucleotide-like code (A,C,G,T,N) may also cause problems.
If you were running a protein blast query and then you want to run a nucleotide query, is better to reset the form (click reset button), to avoid miscalculations.
The BLAST search will not accept "-" in the query. To represent gaps, use a string of N (nucleotides) or X (proteins) instead.
Each sequence must have an identifier, and the identifier must start with ">".
Default expected value (evalue) is 1e-5. Consider to increase this value to get more precise results.
If you upload a fasta file and also paste sequences in the text area, the BLAST search program will only take the uploaded file into count.
Please upload a valid FASTA file or paste a valid sequence in the text area.
There is a problem with the email provided, Please fix it or leave it blank to continue with the BLAST service.
There is a problem with the evalue provided. Please fix it or leave it blank to continue with the BLAST service
There is a problem with the email and evalue provided, Please fix both fields or leave them blank to continue with the service.
You have succesfully submitted a query to the BLAST search service at WeCoNET.